Active Cell Extracts

1) pre-mRNA splicing assays, 2) polyadenylation assays, 3) transcription assays, 4) translation assays,

5) kinase assays, 6) gel shift assays (protein-DNA/RNA interactions), 7) protein-protein interaction assays, and 8) Western blot assays.

Transcription, pre-mRNA capping, splicing, and polyadenylation

Nature Reviews Molecular Cell Biology 7, 391-403 Translation and post-translational modifications, including glycosylation

Also, the active nuclear and cytoplasm extracts can be used for native protein isolation and identification, specifically, as sources of individual cancer biomarkers, tumor suppressors, kinases, cytokines, splicing factors, polyadenylation factors, transcription factors, ubiquitylation/SUMOylation factors, discovery and characterization of disease-related new proteins, protein expression profiles, and protein location studies.

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